Your key to biological relevance

What you study in cells, you will also see in patients.​

Proof of cellular target engagement, the interaction between a molecule and its protein target, is a critical determinant of success in drug discovery.

The effect of a drug depends primarily on the extent of its target engagement, while unwanted side effects are often caused by off-target binding. Therefore, from initial hit discovery to preclinical and clinical investigation, target engagement of drugs should be carefully monitored and controlled.​
The assessment of cellular target engagement using CETSA gives you confidence from biological relevance -compared to traditional biochemical methods, often relying on engineered cellular systems, tagged or truncated proteins in highly defined buffers or modified compounds, which cannot guarantee a translation into desired phenotypic effects.