Martinez Molina, D. et al. Monitoring Drug Target Engagement in Cells and Tissues Using the Cellular Thermal Shift Assay. Science 341, 84-87 (2013) Savitski M. M., Reinhard F. B. M., Franken H., Werner T., Savitski M. F., Eberhard D….
INTRODUCTION With the Cellular Thermal Shift Assay (CETSA®) celebrating its first decade since the PoC publication, the method has gained much interest in the basic science field as well as become an industry standard in applied drug discovery setting…
Download our posters to learn more about CETSA® technology, the science behind it and the case…
Martinez Molina, D. et al. Monitoring Drug Target Engagement in Cells and Tissues Using the Cellular Thermal Shift Assay. Science 341, 84-87 (2013) Savitski M. M., Reinhard F. B. M., Franken H., Werner T., Savitski M. F., Eberhard D….
Read our application notes that we prepared for you to better understand CETSA® technology, the science behind it and the case…
Pelago Bioscience is a Drug Discovery Research Partner supporting preclinical and clinical drug discovery and development. The core of our service is the patented Cellular Thermal Shift Assay (CETSA®) and complementary functional and proteomics assays.CETSA, unlike other solutions on the…
It is a well-established fact in basic science, as well as in drug discovery, that the differences between an assay based on a purified or recombinant protein and a full-length protein in a native cellular context may have substantial impact on the data. This will…
Since the protein targets of any given compound hit from a phenotypic screen are initially unknown, one major challenge with phenotypic-based drug discovery is the need for target identification or deconvolution. Choosing the most efficient and reliable method for…
As a cellular target engagement assay that determines the level of interaction between a ligand and a target protein of interest in a physiologically relevant setting, CETSA® is perfectly suited for hit confirmation. CETSA® requires no protein or ligand modifications…
For a drug to be effective according to the target hypothesis, it must selectively bind to the intended target protein to elicit its effect. Target engagement (TE) is defined as the interaction of ligands with their molecular targets. Assessment of a compound’s TE is…