Uncover How Your Compounds Drive Therapeutic Effects
Understanding a compound’s mechanism of action (MoA) is essential in drug discovery. It reveals how a drug interacts with its target, influences cellular pathways and delivers therapeutic effects. Clarifying these interactions provides crucial insights into a compound’s efficacy, safety, and potential off-target effects, guiding lead optimization and shaping clinical development strategies.
A significant challenge in MoA studies is distinguishing true primary target engagement from downstream biological effects. Without this distinction, there’s a risk of advancing compounds that produce therapeutic outcomes through unintended pathways—potentially compromising safety or efficacy.
We address this challenge through unbiased, proteome-wide profiling powered by CETSA®. This data-driven approach enables a comprehensive understanding of compound-target interactions in a physiologically relevant context. Our methodology helps validate MoA hypotheses, refine drug candidates, and accelerate decision-making throughout preclinical and clinical development.
Key Benefits
- Precise Mechanistic Insight – Distinguish between primary target engagement and downstream effects for greater confidence in therapeutic relevance.
- Comprehensive Interaction Profiling – Gain a clear understanding of compound activity across the proteome in biologically relevant conditions.
- Accelerated Candidate Validation – Make faster, data-driven decisions during preclinical and clinical development.
How It Works
Our MoA service integrates CETSA technology into a structured workflow designed to reveal clear, actionable insights about compound-target interactions:
- Proteome-Wide Interaction Mapping – Unbiased profiling to identify all relevant interactions in a native cellular environment.
- Primary Target Verification – Direct measurement of compound engagement with intended targets, distinguishing it from downstream effects.
- Mechanistic Analysis – In-depth evaluation of pathway modulation to confirm the therapeutic relevance of compound action.
Ready to unlock the full potential of your drug candidates?
Contact Pelago Bioscience today to learn how our CETSA-powered mechanism of action studies can accelerate your path from discovery to development.
Get in touch
Contact us to learn more, to book a meeting or request a quote.