Unravel Molecular Mechanisms in Phenotypic Drug Discovery
One of the most significant challenges in phenotypic drug discovery is target deconvolution—pinpointing the molecular interactions responsible for driving a specific phenotypic response. This understanding is essential for confirming a compound’s mechanism of action, minimizing off-target effects, and ensuring therapeutic relevance.
Traditional target deconvolution methods often fall short, struggling with false positives, indirect interactions, or artificial assay conditions that limit physiological relevance.
These limitations can be overcome using our proteome-wide profiling with CETSA®. This powerful approach allows for a comprehensive analysis of compound-target interactions in conditions that reflect actual cellular environments. Our methodology delivers key insights into target engagement, pathway modulation, and mechanistic action—empowering data-driven decision-making in phenotypic drug discovery tailored to your disease-relevant system.
Key Benefits
- Unbiased Target Identification – Comprehensive, proteome-wide profiling captures direct and indirect interactions in a physiologically relevant context.
- Enhanced Mechanistic Insight – Clarify a compound’s mechanism of action, supporting informed decisions on candidate progression.
- Minimized Off-Target Risks – Detect and reduce potential off-target effects early, improving the likelihood of therapeutic success.
How It Works
Our target deconvolution service leverages CETSA technology for a thorough and scientifically rigorous investigation of compound-target interactions:
- Proteome-Wide Profiling – Unbiased assessment of compound interactions across the proteome within biologically relevant conditions.
- Target Engagement Analysis – Direct measurement of compound binding to reveal primary and off-target interactions.
- Mechanistic Evaluation – In-depth analysis of pathway modulation to confirm mechanisms of action and therapeutic relevance.
Are you looking to uncover the molecular drivers of your phenotypic hits?
Contact Pelago Bioscience today to learn how our target deconvolution services can help you confidently navigate the complexities of drug discovery.
Get in touch
Contact us to learn more, to book a meeting or request a quote.