Validating Efficacy Within Living Systems
In drug discovery, confirming that a compound binds to its intended target within a living organism is crucial for therapeutic efficacy. Without this validation, there is a risk of advancing compounds that fail to perform as expected in complex biological environments, potentially leading to costly setbacks in clinical development.
Unlike traditional assays that rely on indirect measurements, CETSA® provides a direct, accurate assessment of target engagement in vivo. By applying CETSA to various tissues and organs in preclinical animal models or patient-derived material, researchers gain clear insights into whether a drug reaches its intended site of action and engages effectively at clinically relevant concentrations.
This data-driven approach offers a comprehensive view of drug distribution, bioavailability, and on-target interaction within living systems. The result is faster, more confident decision-making—helping you assess efficacy and safety before entering human trials.
Key Benefits
- Direct In Vivo Validation – Accurately measure target engagement within living organisms, providing meaningful data on drug behaviour.
- Enhanced Drug Distribution Insights – Evaluate whether your compound reaches and engages its intended site of action across tissues and organs.
- Accelerated Preclinical Decision-Making – Increase confidence in advancing compounds with proven efficacy and safety profiles before clinical trials.
How It Works
Our in vivo target engagement service integrates CETSA technology into a streamlined, scientifically rigorous process designed to validate compound efficacy within living systems:
- Tissue Sampling and Analysis – Measure direct target engagement in tissues and organs from preclinical animal models or patient-derived materials.
- Distribution and Bioavailability Assessment – Evaluate whether your drug reaches its target site at effective concentrations.
- Data Interpretation for Decision-Making – Analyze engagement data to inform preclinical strategy and assess readiness for human trials.
Need to validate your compound’s target engagement in vivo?
Contact Pelago Bioscience today to learn how our CETSA-powered in vivo studies can help ensure your drug candidate is ready for clinical development.
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Contact us to learn more, to book a meeting or request a quote.