Target Discovery

Our Services Within Target Discovery 

Target Identification

Our CETSA® technology examines how compounds interact with the entire proteome in living cells. This unbiased, whole-proteome profiling gives a clear picture of both direct and off-target effects, as well as the cellular pathways responsible for driving disease, helping you make better, more informed drug discovery decisions from the very start.
Key benefits 

  • Proteome-Wide Insights: Get a complete view of how your compounds engage with proteins across the cell 
  • Early Off-Target Detection: Spot potential issues before they become costly problems 

Pathway Discovery: Identify key cellular pathways influenced by your treatment 

Assay Development

Unlike conventional target engagement assays, CETSA doesn’t require protein or ligand modifications, eliminating time-consuming assay development and molecular biology work, enabling a seamless translation from cells to more complex systems. This means faster setup times, fewer bottlenecks, and quicker access to meaningful data. What you see in cells, you will also see in patients. 

What We Offer 

  • Rapid Assay Setup: Skip the complex probe development—get results faster without the need for cell or protein engineering 
  • Direct Target Engagement Readouts: Measure true interactions within living cells, without artificial modifications 
  • Tailored Assay Design: Customized solutions that adapt to your project’s unique goals and challenges 

Expertise with Difficult Targets: Proven success developing assays for both well-established and hard-to-drug targets 

 Tool Evaluation

At Pelago Bioscience, we specialize in thorough, experimental validation of reference compounds. Our evaluation process confirms that previously reported effects hold under relevant biological conditions—helping you avoid false positives, minimize misleading data, and make more informed decisions as you move forward in your discovery journey. 

Why Choose Our Tool Evaluation Service? 

Stronger Hypothesis Building: Build a solid foundation for exploring target function in disease contexts 

Experimental Validation: Confirm the biological relevance of your tool compounds through rigorous testing 

Reduce Research Risk: Eliminate false positives and misleading data before advancing to lead generation 

Pathway Analysis: Understand how validated compounds influence key biological pathways 

"Pelago Bioscience offers exceptional technical expertise in all aspects of target deconvolution using CETSA – including study design, sample preparation, LC-MS data acquisition, and statistical processing. Our collaboration always proceeded effectively due to Pelago Bioscience's responsiveness, scientific commitment, and dedication to clients resulting in delivery of impactful scientific results."

Benjamin Fontaine, Senior Scientist

LifeMine Therapeutics

"CETSA data is a keystone in our SAR analysis, and by assessing cellular target engagement of our compounds across multiple cell lines we gain confidence in our project prioritizations."

Peter Joyce, CEO and Co-Founder

Grey Wolf Therapeutics

"Our small molecule drug discovery project was seeking a robust assay allowing to screen against the target of interest expressed in its natural cellular environment. Pelago Bioscience's expertise in drug discovery projects, specifically in the CETSA platform and high throughput screens was the key to achieving our goals. We found Pelago's team to be professional, knowledgeable, flexible and dedicated. We highly recommend Pelago Bioscience for drug discovery projects."

Po-Shun Lee, Chief Medical Officer

Generian

"Using our world leading expertise in CETSA we have created a faster and more efficient drug discovery process. We take great pride in knowing our customers are bringing new medicines to market more quickly thanks to our help."

Michael Dabrowski, CEO and Co-founder

Pelago Bioscience